![]() ![]() Digestion of adipose tissue for 45–60 min exclusively results in SVF single cells, whereas a shorter digestion time of only 10 min provides a mixture of single cells and ad-MVF. ĭue to its abundance and minimal-invasive accessibility, adipose tissue is not only an attractive source for the isolation of the SVF but also for the harvesting of adipose tissue-derived microvascular fragments (ad-MVF). The SVF results from the enzymatic digestion of fat samples and is a mixture of endothelial cells, pericytes, smooth muscle cells and stem cells. Taking this into account, the stromal vascular fraction (SVF) of adipose tissue is frequently used to induce the formation of microvascular networks. However, blood vessels do not only consist of one specific cell type but exhibit a complex composition with an inner endothelial lining and surrounding vessel wall-stabilizing cell layers. Ī common prevascularization strategy is the seeding of vessel-forming cells, such as endothelial cells or stem cells, onto scaffolds. the creation of preformed microvascular networks in scaffolds prior to their implantation, has emerged as a promising concept. To achieve this, prevascularization, i.e. Moreover, they should rapidly vascularize to ensure a sufficient oxygen supply and, thus, cellular survival. To ideally promote the function and regenerative capacity of seeded cells, scaffolds should mimic the natural extracellular matrix. A well-established approach for the generation of tissue constructs is the seeding of cells onto different biomaterials, which serve as three-dimensional scaffolds. Tissue engineering is an interdisciplinary field of biomedical research focusing on the restoration of tissue defects or even on the replacement of complete organs. The present study demonstrates that ad-MVF are highly potent vascularization units that markedly accelerate and improve scaffold vascularization when compared to the SVF. In contrast, non-seeded matrices exhibited a poor vascularization, incorporation and epithelialization over time. This was associated with an enhanced cellular infiltration, collagen content and density of CD31 +/GFP + microvessels particularly in the center of the implants, demonstrating a better incorporation into the surrounding host tissue. After in vivo implantation, the vascularization of ad-MVF-seeded scaffolds was improved when compared to SVF-seeded ones, as indicated by a significantly higher functional microvessel density. While both isolates contained a comparable fraction of endothelial cells, perivascular cells, adipocytes and stem cells, ad-MVF exhibited a significantly higher viability. Moreover, collagen-glycosaminoglycan matrices (Integra®) were seeded with identical amounts of the isolates and implanted into full-thickness skin defects within dorsal skinfold chambers of GFP − recipient mice for the intravital fluorescent microscopic, histological and immunohistochemical analysis of implant vascularization and incorporation throughout an observation period of 2 weeks. SVF single cells and ad-MVF were enzymatically isolated from epididymal fat pads of green fluorescent protein (GFP) + donor mice to assess their viability and cellular composition using fluorescence microscopy and flow cytometry. Therefore, we herein hypothesized that the ad-MVF-based prevascularization of scaffolds is superior to the conventional SVF single cells-based approach. In contrast to SVF single cells, they represent a mixture of intact arteriolar, capillary and venular vessel segments. Alternatively, adipose tissue-derived microvascular fragments (ad-MVF) may serve as vascularization units. The seeding of scaffolds with the stromal vascular fraction (SVF) of adipose tissue is a common prevascularization strategy in tissue engineering. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |